BlueScreen HC: The luminescent adaptation of GreenScreen

BlueScreen HC: The luminescent adaptation of GreenScreen
Click to Playtemp-thumb

Gentronix Ltd.

BlueScreen HC is based on the fast, accurate GreenScreen HC genotoxicity assay from Gentronix Ltd. BlueScreen HC uses the same human-derived, p53-competent TK6 cells to host a patented reporter ...read more

Request Pricing





Receive your quote directly from Gentronix Ltd. for BlueScreen HC: The luminescent adaptation of GreenScreen

Description

 

BlueScreen HC is based on the fast, accurate GreenScreen HC genotoxicity assay from Gentronix Ltd.

BlueScreen HC uses the same human-derived, p53-competent TK6 cells to host a patented reporter system that exploits the proper regulation of the GADD45a gene. However, in place of GFP, BlueScreen HC utilizes a luciferase from the marine copepod Gaussia princeps to generate a luminescent output.

The BlueScreen luminescent assay delivers both high specificity and high sensitivity and detects all common mechanistic classes of genotoxin, just like GreenScreen HC. The 96-well microplate format of BlueScreen is rapid to set up and results are generated after 48 hours’ incubation. A single microplate is sufficient for the simultaneous testing of 8 compounds and the protocol is readily automated using standard laboratory equipment. The superior signal-to-noise ratio makes it more suitable for adaptation to even higher throughput screening in 384-well format. The BlueScreen luminescent output also circumnavigates issues of test compound autofluorescence.

Key Principles of BlueScreen:

GADD45a plays an important role in mediating the adaptive response to genotoxic stress. The patented system incorporates complex regulatory elements to enable a faithful GADD45a response. The assay generates positive results for direct-acting mutagens, clastogens, as well as aneugens, and topoisomerase and polymerase inhibitors. Importantly, correct negative results are produced for non-carcinogens, many of which give misleading positive results in other in vitro genotoxicity tests. A metabolic activation protocol using rodent S9 liver extract extends the range of compounds detected to include genotoxic metabolites.

Bluescreen Assay Protocol:
BlueScreen HC uses a single cell strain, which permits 8 compounds to be tested across 8 dilutions (2-fold serial), together with untreated and positive controls, in one 96-well microplate. After arraying test compounds and dilutions, growing cells are added to each well. For studies without S9 metabolic activation data are collected after incubation (48h) using a multimode microplate reader. For studies incorporating S9 metabolic activation, cells are washed after a 3h incubation with test compound and S9. Measurements are made by microplate reader after a further 45h incubation period. Simple software gives automated decisions and a clear graphical output, including a complimentary indication of cytotoxicity (RCD).

Feedback

Request info

To:Gentronix Ltd.
From:
Please contact me / call me with the BlueScreen HC: The luminescent adaptation of ...,

I am looking to purchase in /

.
About you

Write a review

Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).

  
Your Email

 



Ease of use

After sales services

Value for money

WATCH:
The benefits of becoming a reviewer
Share your opinions...()
HINTS & TIPS
Give reasons for the star ratings you have awarded for each area.
Would you recommend this to a colleague?
What would you like to have known about equipment and consumables before you purchased?



Why make an enquiry with SelectScience?

  • EASY It’s quick and simple to do
  • FAST Your enquiry will be delivered straight to the manufacturer
  • FREE You’re under no obligation
  • SECURE We only pass your details on to trusted suppliers at your request
  • SAVE TIME Submit your details once and make multiple enquiries
SelectScience Trusted Banner