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Status:
Reviewer
Member since: 2019
Organization: Facultatea De ?tiin?a ?i Tehnologia Alimentelor Cluj-Napoca
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- Ease of use
- After sales service
- Value for money
Great results. An easy way to purify proteins.
Application Area: Protein purification
"It was an effective dye-ligand affinity chromatography which helped me purify the proteins of interest. The instructions were effective in helping me choose the right conditions for purifying the proteins from a mixture."
Review date: 15 Apr 2019 | Blue Sepharose 6 Fast Flow
Status:
Advanced Reviewer Member since: 2015
Organization: UNESP
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- Ease of use
- After sales service
- Value for money
Greatest results
Application Area:Protein Purification
""Blue Sepharose 6 Fast Flow binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The binding capacity of this resin is 18 mg/ml. The bind proteins can be eluted either by changing pH or using salt concentration gradient. I recommend this resin to be used for protein purification. ""
Review date: 16 Aug 2016 | Blue Sepharose 6 Fast Flow
Status:
Expert Reviewer Member since: 2012
Organization: UNESP/IBILCE
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- Ease of use
- After sales service
- Value for money
Application Area:Protein Purification
"Blue Sepharose 6 Fast Flow binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The binding capacity of this resin is 18 mg/ml. The bind proteins can be eluted either by changing pH or using salt concentration gradient. I recommend this resin to be use for protein purification. "
Review date: 29 May 2014 | Blue Sepharose 6 Fast Flow
Blue Sepharose 6 Fast Flow is a member of the GE Healthcare range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Features:
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
- BioProcess medium supported for industrial applications and well-established in approved processes
