Status:
ReviewerMember since: 2019
Organization: Facultatea De ?tiin?a ?i Tehnologia Alimentelor Cluj-Napoca
Great results. An easy way to purify proteins.
Application Area: Protein purification
"It was an effective dye-ligand affinity chromatography which helped me purify the proteins of interest. The instructions were effective in helping me choose the right conditions for purifying the proteins from a mixture."
Status:
Advanced ReviewerMember since: 2015
Organization: UNESP
Greatest results
Application Area:Protein Purification
""Blue Sepharose 6 Fast Flow binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The binding capacity of this resin is 18 mg/ml. The bind proteins can be eluted either by changing pH or using salt concentration gradient. I recommend this resin to be used for protein purification. ""
Status:
Expert ReviewerMember since: 2012
Organization: UNESP/IBILCE
Application Area:Protein Purification
"Blue Sepharose 6 Fast Flow binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The binding capacity of this resin is 18 mg/ml. The bind proteins can be eluted either by changing pH or using salt concentration gradient. I recommend this resin to be use for protein purification. "
Blue Sepharose 6 Fast Flow is a member of the GE Healthcare range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
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