Blue Sepharose 6 Fast Flow

Manufacturer GE Healthcare
4.3
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5.0
  |  1 reviews


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Average Rating: 4.3
1 Scientist has reviewed this product

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Rating: 4.3

  • Application Area: Protein Purification

"Blue Sepharose 6 Fast Flow binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+. The binding capacity of this resin is 18 mg/ml. The bind proteins can be eluted either by changing pH or using salt concentration gradient. I recommend this resin to be use for protein purification. "

Review date: 29 May 2014 | Blue Sepharose 6 Fast Flow
Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale.

Blue Sepharose 6 Fast Flow is a member of the GE Healthcare range of affinity chromatography media for capture and intermediate purification.

Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.

The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.


Features:

  • Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
  • Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
  • BioProcess medium supported for industrial applications and well-established in approved processes