BIOTAQ™ DNA Polymerase

 4.3/5.0 (1 review)

Bioline UK

BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent. BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield ...read more

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Reviews

Van Reese


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Member since 2002

Organization:
University of Utah- Vascular Lab
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 29 Aug 2003

BIOTAQ™ DNA Polymerase

"We have used this polymerase for several years with excellent results. We have tried several of the other DNA polymerases, but this give the best yield."

Description

 

BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.

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