This column-based procedure removes salts, proteins, nucleotides and small oligos from the upstream enzymatic reactions. The improved formulation of the binding buffer and wash solution allows higher yield of purified cDNA product compared to an earlier version of the kit. The cDNA is purified in three steps that consist of (1) mixing with binding buffer and adding to the column, (2) washing with wash buffer, and (3) elution in a minimum of 6 µL of water. This procedure can be used for purifying a maximum of 5 µg cDNA material ranging in size from 75 bp to 23 kb.
The cDNA purification kit provides a fast, efficient and convenient method for purification of double stranded cDNA.