Bio-Plex Pro Magnetic Cell Signaling Assays are multiplexed immunoassays for the detection of intracellular phosphoproteins and total target proteins in cell and tissue lysates. These assays utilize tailored antibodies bound on 6.5 µm magnetic beads. Bio-Plex Pro total target assays measure the total abundance of each target protein, regardless of its phosphorylation state. Bio-Plex Pro phosphoprotein assays measure the relative amounts of phosphorylated proteins only. When both phosphoprotein and total protein assays are performed, they must be run in different wells due to shared bead regions. Using the multiplex format, the profiles of multiple phosphorylated proteins involved in cell signaling pathways can be detected using only 50 µl of lysate. Features and Benefits of Magnetic Cell ...Read more
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