Bio-Monolith Protein A and Protein G by Agilent Technologies

Agilent Bio-Monolith Protein A and Protein G LC columns are designed for analytical separation of all IgG subclasses (human and mouse). They deliver fast and accurate quantitation of mAb titer and purification of small amounts of mAb for further characterization.

These monolithic columns enable extremely fast separations to speed up method development time and decrease costs. They are compatible with HPLC and UHPLC systems, including the Agilent 1100, 1200, and 1260 Bio-inert Quaternary LC.

Features:

• Analytical separation of all IgG subclasses (human and mouse), Protein G offers alternate selectivity for those IgG molecules that do not bind to Protein A
• Flow-rate independent separations
• no diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid
• Extremely fast separations speed up method development time and decrease costs
• Locking in method parameters takes significantly less time and buffer
• Key applications are quantitative determination of IgG (fermentation titer calculation)