HiTrap Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.
Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.Benzamidine Sepharose 4 Fast Flow Features:
For convenient, one-step r... Read more
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Benzamidine Sepharose 4 Fast Flow
Average Rating: 4.3
1 Scientist has reviewed this product
Status:
Expert Reviewer
Member since: 2012
Organization: UNESP/IBILCE
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Rating: 4.3
Application Area: Protein Purification
"Benzamidine Sepharose 4 Fast Flow (high sub) resin used in affinity chromatography. This resin has covalently attached benzamidine which is an inhibitor for serine proteinases. The mixture of proteins that contain serine proteinases are passed through this resin at pH 7.5-8.5. The bound proteins are eluted with buffer pH 3.0. This is an old method which has some drawbacks as some proteins like Phospholipases may also bind to this resin. In a new method that we have developed after applying the sample in buffer containing 100mM salt, the non-specific proteins are eluted with the same buffer with an additional 500mM salt. Lastly the serine proteinases are eluted by decreasing the pH to 3.0. This method has improved the affinity based purification procedure. I recommend this method for purification of serine proteinases by Benzamidine Sepharose 4 Fast Flow (high sub). The resin can be stored in 20% ethanol after use."
Review date: 07 Jan 2014 | Benzamidine Sepharose 4 Fast Flow
HiTrap Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.
Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
Benzamidine Sepharose 4 Fast Flow Features:
- For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
- p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up.
- Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity
