Nextera DNA Sample Preparation Kits

Nextera DNA Sample Preparation Kits
 4.3/5.0 (4 reviews)

Illumina

Nextera DNA Sample Preparation kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. ...read more

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Reviews

Prashant Patil


Status:


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Member since 2013

Job Title:
PhD Student
Discipline:
Biology
Work Field:
Government / Military
Organization:
CSIR-Institute Of Microbial Technology
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RATING: 3.7

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 14 Jan 2014

Application Area: Bacterial PathoGenomics
Nextera DNA Sample Preparation Kits

"The fastest and easiest library preparation for illumina sequencing which is compatible with the small genomes. One great advantage over the True Seq kits is very low input DNA required, 1ng, and easy normalization. However it required optimization for high GC and low GC content genomes."

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Xinping Yang


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Member since 2011

Organization:
NIH- NIDCD
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 11 Jul 2011

Nextera DNA Sample Preparation Kits

"So far, we are very satisfied with its performance."

SARAH KLEINSTEIN


Status:


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Member since 2011

Organization:
FRED HUTCHINSON CANCER RES. CTR.
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 04 Jul 2011

Nextera DNA Sample Preparation Kits

"Wonderful product, although it can be picky about what types of files you upload."

Di Zhang


Status:


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Member since 2007

Organization:
MD Anderson Cancer Center
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 11 Jun 2007

Nextera DNA Sample Preparation Kits

Description

 

Nextera DNA Sample Preparation kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

The TruSeq Dual Index Sequencing Primer Box is required for single-read and paired-end sequencing of Nextera libraries on HiSeq 1000/2000, HiScanSQ, and Genome Analyzer IIx instruments. These primers are required for non-indexed, single-indexed, and dual-indexed Nextera libraries.



Please note that a Nextera Index kit (FC-121-1011 or FC-121-1012) is required to complete the protocol, regardless of the sample pooling level used for sequencing.

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