Status:
Expert ReviewerMember since: 2017
Organization: Roche
Best flow cytometry system
Application Area: flow cytometry
"I really like LSRII from BD. The system is very reliable, easy to use and the data is very reproducible"
Status:
ReviewerMember since: 2016
Organization: McMaster University
Good results, dependable overall.
Application Area:Flow cytometry to assess immune cells in blood samples
"This machine works best in a room that is cooler than typical room temperature. It’s very large but typically dependable. Has less laser colours and features compared to newer cytometers like the BD fortessa. This machine is easy to get bubbles or clots in, under which it is easy to lose your sample, which is not the best."
Status:
ReviewerMember since: 2020
Organization: Universidad Padova
Easy to use, great results
Application Area:Research Cell
"This advanced instrument is ideal to reach the research goals faster, more reliable and it is easy to use. You can save time with flow cytometry protocols compared to other molecular techniques. The sample preparation takes a very short time, after setting up the protocol, you can analyze a high number of samples in a short time"
Status:
Advanced ReviewerMember since: 2012
Organization: Mater Research Institute
"I have a STD 4 laser, 10 color instrument, and have been running it for almost a decade. Currently, I have just updated to DiVa 8, the latest generation UV laser and a solid state violet laser. My only real issue is that of all the instruments in my flow core this one blocks the most frequently, but it has the highest use (150-160 hrs/month). I now have a second SIP, and swap SIPs when blockages are reported. The major issue here is sample preparation rather than a defined fault in the instrument. New technology instruments (3 laser, 13 color @ ~AU$100-120,000) means this instrument is struggling as far as value for money is concerned. "
Status:
ReviewerMember since: 2013
Organization: Stanford Univ. Herzenberg Laboratory
Application Area:Immunophenotyping
"A great analytical tool for cell biology. When used correctly it gives a lot of valuable data and insight into research questions"
Status:
ReviewerMember since: 2013
Organization: NATIONAL CANCER INSTITUTE
Application Area:immunology
"This flow cytometer has good spectral separation. I found that it is excellent for 9 or more flourophores. It gives a fast analysis with very good accuracy. Reproducibility between experiments is excellent."
Status:
ReviewerMember since: 2013
Organization: John Hopkins University
Application Area:tumor immunology
"The BD LSR II System is an excellent tool for multi-color flow cytometry. BD FacsDiva software is very user friendly and easy to navigate."
Status:
ReviewerMember since: 2013
Organization: Kyowa Hakko Kirin California, Inc.
Application Area:cell based assays
"I love using the LSR 2, though analysis can be complicated when doing 8 colors or more. It allows a lot of analysis with just one sample."
Status:
ReviewerMember since: 2012
Organization: University of Nebraska-Lincoln
Application Area:Flow Cytometry
"After several years on the market this is still the most versatile flow cytometer available. The software for both acquisition and analysis of results is user-friendly. The wide variety of configuration can be used to analyse more than 10 distinct fluorophores."
Status:
ReviewerMember since: 2012
Organization: Allergan
Application Area:Cell Analysis
"The equipment is pretty easy to use because of the integration between software and hardware. The data derived from the machine is pretty accurate compared to other cellular assays. However, the kit for the calibration of the baseline fluorescence is relatively expensive."
Four-laser, 18-color benchtop flow cytometer
Building on the ease of use and flexibility of the BDTM LSR, the first UV benchtop analyzer, BD Biosciences introduces the BDTM LSR II. The BD LSR II has performance, capabilities, and flexibility never seen before in a benchtop analyzer. Free up your high-performance cell sorter by moving your application(s) that require a UV laser to the BD LSR II.
The BD LSR II can be configured with up to four fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. The BD LSR II can be configured with a 488 nm, 405 nm, 633 nm, and 355nm UV laser. At the heart of the BD LSR II are new digital electronics. The electronics digitize signals 10 million times per second in 16,384 discrete levels. This expands channel resolution and increases detection sensitivity. Electronic dead time is eliminated; more precise fluorescent measurements can be made improving linearity, compensation, and quantitation of fluorescence measurements.
BD LSR II System
Laser Options