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3 Scientists have reviewed this product
5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
"Very quick and easy to use. Low inner- and inter-assay variability. Highly recommend it!"
Review date: 26 May 2015 |
BCA Protein Assay Reagent (bicinchoninic acid) Rating: 5.0
"It is the most comfortable method for analysis of proteins, and very essay to prepare solution provided in kit, with proper guidance provided in the manual. It is good for test tube methods as well as for microplate methods. "
Review date: 18 Feb 2015 |
BCA Protein Assay Reagent (bicinchoninic acid) Rating: 4.3
Review date: 02 Feb 2010 | BCA Protein Assay Reagent (bicinchoninic acid)
Used in more labs than any other detergent-compatible protein assay.
The Thermo Scientific Pierce BCA Protein Assay remains one of the most popular protein assay methods worldwide. The detergent-compatible method provides for accurate determination of protein concentration with most sample types encountered in protein research laboratories. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
Highlights include:
• Colorimetric method; read at 562 nm
• Compatible with most ionic and nonionic detergents
• Faster and easier than the Lowry method
• Working reagent stable for 24 hours
• Linear working range for BSA from 20-2,000 µg/ml
• Minimum detection level of 5 µg/ml with the enhanced protocol
• Adaptable to microplates
• Less protein-to-protein variation than dye-binding methods
BCA Protein Assay Applications:
• Studying protein:protein interactions
• Measuring column fractions after affinity chromatography
• Estimating percent recovery of membrane proteins from cell extracts
• High-throughput screening of fusion proteins