B-Raf Kinase Cascade Assay Kit

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B-Raf Kinase Cascade Assay Kit
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The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.

Receive your quote directly from EMD Millipore, a division of Merck KGaA, Darmstadt, Germany.

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Description

The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.






Receive your quote directly from EMD Millipore, a division of Merck KGaA, Darmstadt, Germany for B-Raf Kinase Cascade Assay Kit