The B Cell Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched B cells from human PBMCs. Non-B cells, i.e., T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells, are indirectly magnetically labeled using a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Highly pure B cells are obtained by depletion of magnetically labeled non-B cells.
For evaluation of MACS® Separations, staining with fluorochrome-conjugated Anti-Biotin antibodies (Anti-Biotin-PE or -APC) and CD19 antibodies is recommended.
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