AxyPrep™-96 PCR Cleanup Kit



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This kit employs a special Binding Solution in combination with an optimized 96-well plate to purify linear DNA fragments in a high-throughput format.

This product is designed to purify DNA fragments longer than 75 bp from PCRs and other enzymatic reactions, with an expected recovery of 70-90%. Each well has a binding capacity of up to 8 µg and the plate can be used with either a vacuum manifold or centrifuge. It is not necessary to remove mineral oil overlays from the PCRs before purification. This protocol will remove unincorporated primers (<50 nt), enzymes and unlabeled and fluorescent dye-labeled mononucleotides. The purified DNA fragments are suitable for a variety of applications, such as sequencing, ligation, restriction analysis, in vitro transcription, microinjection and microarrays.

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AxyPrep™-96 PCR Cleanup Kit

Manufacturer Axygen Scientific, Inc.

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