Automated System for BigDye® Terminator Cleanup

Automated System for BigDye® Terminator Cleanup
 4.3/5.0 (1 review)

HAMILTON Robotics

Removal of unincorporated dye terminator after DNA sequencing reactions is a prerequisite to load samples on automated capillary sequencers. For rapid and effective removal of unincorporated dye termi...read more

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Reviews

Thando Ndlovu


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Member since 2010

Organization:
University of Stellenbosch
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 09 Apr 2014

Application Area: Bacteriology In Environmental Samples
Automated System for BigDye® Terminator Cleanup

"It gives the best results in a short time. "

Description

 

Removal of unincorporated dye terminator after DNA sequencing reactions is a prerequisite to load samples on automated capillary sequencers. For rapid and effective removal of unincorporated dye terminator, Agencourt CleanSEQ® uses Solid Phase Reversible Immobilization (SPRIT) of DNA products on magnetic microparticles.

The kit has been automated on the HAMILTON MICROLAB® STAR. The flexibility of the STAR and the robustness of the method facilitate implementation in low or high throughput environments.

The application was optimized to attain:
• minimal costs for reagents and consumables
• high quality data
• maximum read length

Results generated on the STAR compare favorably with results from manual processing. Automation of other kits from Agencourt on the same system is easily done due to the flexibility of the STAR.

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