Removal of unincorporated dye terminator after DNA sequencing reactions is a prerequisite to load samples on automated capillary sequencers. For rapid and effective removal of unincorporated dye terminator, Agencourt CleanSEQ® uses Solid Phase Reversible Immobilization (SPRIT) of DNA products on magnetic microparticles. The kit has been automated on the HAMILTON MICROLAB® STAR. The flexibility of the STAR and the robustness of the method facilitate implementation in low or high throughput environments. The application was optimized to attain: • minimal costs for reagents and consumables • high quality data • maximum read lengthResults generated on the STAR compare favorably with results from manual processing. Automation of other kits from Agencourt on the same system is easily done due...Read more
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Member since: 2010
Organization: University of Stellenbosch
The application was optimized to attain: