Automated System for BigDye® Terminator Cleanup
Removal of unincorporated dye terminator after DNA sequencing reactions is a prerequisite to load samples on automated capillary sequencers. For rapid and effective removal of unincorporated dye terminator, Agencourt CleanSEQ® uses Solid Phase Reversible Immobilization (SPRIT) of DNA products on magnetic microparticles.
The kit has been automated on the HAMILTON MICROLAB® STAR. The flexibility of the STAR and the robustness of the method facilitate implementation in low or high throughput environments.
The application was optimized to attain:
Results generated on the STAR compare favorably with results from manual processing. Automation of other kits from Agencourt on the same system is easily done due to the flexibility of the STAR.