ATPliteTM 1step is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is an alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
ATPlite 1step is a true homogeneous high sensitivity ATP monitoring 1-step addition assay kit for the quantification of viable cells. The kit can be used for continuous process systems such as in-line systems in high throughput environments. These in-line systems do not require a long signal half-life since the time between addition of the reagent and reading the resulting luminescence is relatively short (minutes). The decrease of the luminescent light-output is approximately 15% after 30 minutes. This decrease is independent of cell numbers but may differ between cell type and medium. The maximum cell number that can be applied has been determined to be 50,000 cells per well for 96-well and 12,500 cells per well for 384-well microplates. Because the kit needs no stabilization of the luminescence signal, high throughput is preserved.
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