Anti-rat Ig HRP detection kit by BD Biosciences

The BD Pharmingen™ Anti-Ig HRP Detection Kits are designed for immunohistochemical staining of acetone-fixed frozen sections, formalin- or zinc-fixed paraffin sections, and cytospins/smears. The detection kit can be used with any purified primary antibody. Each kit includes a biotinylated anti-Ig antibody that is species-specific (anti-mouse, anti-rat or anti-hamster), antibody diluent, pre-diluted Streptavidin-horseradish peroxidase (SAv-HRP), and DAB substrate. The affinity-purified, polyclonal, biotinylated secondary antibody is adsorbed against multiple sera of related species, reducing non-specific staining, while providing maximum reactivity. Antibody diluent is a buffered solution containing
protein stabilizers to enhance antibody reactivity, detergent for permeabilization, and blocking agents to reduce non-specific staining. The ultra-sensitive SAv-HRP is pre-diluted for convenience. DAB substrate is provided for signal development. Consistent results are assured with all these matched reagents. A comprehensive series of protocols, for immunostaining in different types of tissues, is provided with the kit.