This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine in a strong acid solution to form a ureido group adduct. This ensures... Read more
The supplier does not provide quotations for this product through SelectScience. Browse these related products instead
Related Products
.jpg.ashx?width=100&height=100&bgcolor=white)
Anti-Citrulline (Modified) Detection Kit
The supplier does not provide quotations for this particular product through SelectScience. You can search for similar products in our product directory.
Average Rating: 4.3
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2022
Organization: Novo Nordisk A/S
-
- Ease of use
- After sales service
- Value for money
Very good product
Rating: 4.3
Application Area: Provides reagent for the modification and detection of citrulline containing proteins by western blo
"The product was quite useful in application and was useful in western blotting"
Review date: 29 Jul 2022 | Anti-Citrulline (Modified) Detection Kit
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine in a strong acid solution to form a ureido group adduct. This ensures detection of citrulline-containing proteins regardless of neighboring amino acid sequences. Detection of the modified citrulline proteins is subsequently performed using a standard immunoblot protocol with the anti-Citrulline and goat anti-rabbit IgG-HRP.
