Applied Biosystems® AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation.
- Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
- Time-release thermal activation improves sensitivity in low copy number amplifications
- Successful multiplex PCR saves time and reagents
- Includes GeneAmp® 10X PCR Buffer II and 25 mM MgCl2 Solution
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques. Higher Specificity, Higher Yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-release manner during the denaturation steps of thermal cycling. With or without a View Morelimited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.