AMP2/GMP2 TR-FRET Assay by BellBrook Labs
Manufacturer BellBrook Labs
Direct AMP/GMP Detection method with a TR-FRET readout.
Homogenous detection of AMP or GMP producing enzymes (e.g. ligases, synthetases, phospodiesterases) is now possible with a TR-FRET readout. Like the original, FP-based assay the Transcreener® AMP2/GMP2 TR-FRET assay is designed for automated HTS platforms:run your enzyme reaction, add detection reagents, and read the far red TR-FRET signal on any multimode plate reader using the same filter sets as HTRF®.XMP enzyme products, including AMP,... Read more
AMP2/GMP2 TR-FRET Assay
Direct AMP/GMP Detection method with a TR-FRET readout.
Homogenous detection of AMP or GMP producing enzymes (e.g. ligases, synthetases, phospodiesterases) is now possible with a TR-FRET readout. Like the original, FP-based assay the Transcreener® AMP2/GMP2 TR-FRET assay is designed for automated HTS platforms:run your enzyme reaction, add detection reagents, and read the far red TR-FRET signal on any multimode plate reader using the same filter sets as HTRF®.
XMP enzyme products, including AMP,GMP and CMP displace a fluorescent tracer from a highly selective antibody resulting in a decrease in FRET. The time gated detection and far red tracer reduce compound interference and provide a robust ratiometric readout.
AMP2/GMP2 TR-FRET Assay Targets:
Homogenous detection of AMP or GMP producing enzymes (e.g. ligases, synthetases, phospodiesterases) is now possible with a TR-FRET readout. Like the original, FP-based assay the Transcreener® AMP2/GMP2 TR-FRET assay is designed for automated HTS platforms:run your enzyme reaction, add detection reagents, and read the far red TR-FRET signal on any multimode plate reader using the same filter sets as HTRF®.
XMP enzyme products, including AMP,GMP and CMP displace a fluorescent tracer from a highly selective antibody resulting in a decrease in FRET. The time gated detection and far red tracer reduce compound interference and provide a robust ratiometric readout.
AMP2/GMP2 TR-FRET Assay Targets:
- Ligases: Ub and SUMO Ligases, DNA and RNA Ligases
- Synthetases: Acyl CoA Synthetase, Amino-Acyl tRNA Synthetases, NAD Synthetase
- Phosphodiesterases: cAMP, cGMP
- Sialyltransferases: (CMP detection)
- Protein deAMPylase
- SINGLE ADDITION: one-step, simple mix and read format.
- ROBUST: Z’ > 0.7 for initial velocity reactions
- LESS INTERFERENCE: Far red fluorescence minimizes compound interference.
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