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Member since: 2012
Application Area: Protein Purification And Concentration
"Amicon® Ultra-4 Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins below 3 kDa. This filter device can be used to concentrate a pure protein with molecular mass above 3 kDa. The recommended velocity to spin this device is 5000 RPM, however when the filter is used for long time it may be useful to decrease this speed to 3000 RPM as there is always chance of loss of proteins after using it for a long time. The second more important thing is to fill this device to a maximum of 3.5 ml as there is a chance of filter loss above this volume.This is suitable for small scale protein concentration. I recommend it to use for protein separation and concentration and to store this device either in 20% Ethanol or 0.02% Na-azide solution."