Amicon® Ultra-4 Centrifugal Filter Units

Amicon® Ultra-4 Centrifugal Filter Units
 4.3/5.0 (1 review)

EMD Millipore, a division of Merck KGaA, Darmstadt, Germany

• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs • High retentate recovery of >90% • Vertical membrane reduces concentration polarization for ultra-fast ...read more

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Reviews

Anwar Ullah


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Member since 2012

Job Title:
Post Doc / Research Fellow
Discipline:
Biochemistry
Work Field:
Academia / University
Organization:
UNESP/IBILCE
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Jan 2014

Application Area: Protein Purification And Concentration
Amicon® Ultra-4 Centrifugal Filter Units

"Amicon® Ultra-4 Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins below 3 kDa. This filter device can be used to concentrate a pure protein with molecular mass above 3 kDa. The recommended velocity to spin this device is 5000 RPM, however when the filter is used for long time it may be useful to decrease this speed to 3000 RPM as there is always chance of loss of proteins after using it for a long time. The second more important thing is to fill this device to a maximum of 3.5 ml as there is a chance of filter loss above this volume.This is suitable for small scale protein concentration. I recommend it to use for protein separation and concentration and to store this device either in 20% Ethanol or 0.02% Na-azide solution."

Description

 

• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
• High retentate recovery of >90%
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
• Heat-sealed membrane minimizes downstream extractables
• 100% integrity tested for reliable performance
• Convenient sample monitoring with translucent housing and volume gradations
• Direct pipette sample access eliminates processing step to recover concentrate
• High concentration factors of 80–100X

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