Amersham Protran™ (formally Whatman Protran) nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible.
Protran membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent.
Unlike PVDF membranes, Protran nitrocellulose does not require a methanol prewetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments.
Amersham™ Protran Nitrocellulose Membranes Features:
- High binding, low background - The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.
- High retention of small proteins - high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through’.
- Protran blotting sandwiches - A precut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time.