Status:
ReviewerMember since: 2018
Organization: LSUHSC
very best product
Application Area: Mouse Retina tissue WB
"This product is very good as compared to other company product. I have tried 6 different company product. this is best"
Status:
ReviewerMember since: 2016
Organization: Case Western Reserve University
Great product
Application Area:Western blotting
"As a regular user of western blot reagents, I find the ECL primer extremely sensitive and easy to use."
Status:
Advanced ReviewerMember since: 2010
Organization: Lovelace Respiratory Research Institute
Application Area:Protein Western Blotting
"It is relatively easy to use but don’t use it in a brightly lit room."
Status:
ReviewerMember since: 2011
Organization: Lehigh University
Application Area:Cell Biology
"Very easy to use and make. Easy directions within the kit. Can be finicky in presence of light."
Status:
ReviewerMember since: 2011
Organization: Mahidol University
Application Area:Neuroscience
"This kit is a high sensitivity ECL. Sometimes we dilute it in distilled water before pouring on to the membranes. It needs incubating for 5 minutes and that's it."
Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. Suitable for use with film of CCD camera and a low pg detection level.
The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
Features
• High signal intensity and sensitivity allow the use of highly diluted primary and secondary antibodies with no reduction in sensitivity
• Stable signal allows multiple exposures and large experimental series
• Optimized for use with CCD-based imagers such as ImageQuant™ LAS 4000
• Simple to adapt to established laboratory protocols due to compatibility with most commercially available Western blotting reagents such as molecular weight markers, membranes, and blocking reagents