Amersham™ ECL™ Prime Western Blotting Reagent by Cytiva

Manufacturer Cytiva
4.3
/
5.0
  |  7 reviews
Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. Suitable for use with film of CCD camera and a low pg detection level.The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significant... Read more
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Amersham™ ECL™ Prime Western Blotting Reagent by Cytiva product image
Amersham™ ECL™ Prime Western Blotting Reagent



Average Rating: 4.3
7 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: LSUHSC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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very best product
Rating: 4.0

  • Application Area: Mouse Retina tissue WB

"This product is very good as compared to other company product. I have tried 6 different company product. this is best"

Review date: 25 Jul 2018 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Case Western Reserve University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product
Rating: 5.0

  • Application Area:Western blotting

"As a regular user of western blot reagents, I find the ECL primer extremely sensitive and easy to use."

Review date: 22 Dec 2016 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2010

  • Organization: Lovelace Respiratory Research Institute



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.0

  • Application Area:Protein Western Blotting

"It is relatively easy to use but don’t use it in a brightly lit room."

Review date: 25 Apr 2014 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Lehigh University



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Rating: 3.7

  • Application Area:Cell Biology

"Very easy to use and make. Easy directions within the kit. Can be finicky in presence of light."

Review date: 19 Jan 2012 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Mahidol University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Rating: 4.7

  • Application Area:Neuroscience

"This kit is a high sensitivity ECL. Sometimes we dilute it in distilled water before pouring on to the membranes. It needs incubating for 5 minutes and that's it."

Review date: 12 Jan 2012 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UNIV. OF PUERTO RICO CANCER CTR.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0
"Very easy to use. Incubation of only one minute. Low background."

Review date: 04 Jul 2011 | Amersham™ ECL™ Prime Western Blotting Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Erasmus Medical Center



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Rating: 3.7
"Kit is very self explanatory. Very detailed instruction sheet."

Review date: 21 Jan 2011 | Amersham™ ECL™ Prime Western Blotting Reagent

Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. Suitable for use with film of CCD camera and a low pg detection level.

The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Features
• High signal intensity and sensitivity allow the use of highly diluted primary and secondary antibodies with no reduction in sensitivity
• Stable signal allows multiple exposures and large experimental series
• Optimized for use with CCD-based imagers such as ImageQuant™ LAS 4000
• Simple to adapt to established laboratory protocols due to compatibility with most commercially available Western blotting reagents such as molecular weight markers, membranes, and blocking reagents

Product Overview

Amersham™ ECL™ Prime Western Blotting Reagent by Cytiva product image

Amersham™ ECL™ Prime Western Blotting Reagent

Manufacturer Cytiva

4.3 / 5.0 | 7 reviews