Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumpti...read more
"Kit is very self explanatory. Very detailed instruction sheet."
Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. Suitable for use with film of CCD camera and a low pg detection level.
The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
Features • High signal intensity and sensitivity allow the use of highly diluted primary and secondary antibodies with no reduction in sensitivity • Stable signal allows multiple exposures and large experimental series • Optimized for use with CCD-based imagers such as ImageQuant™ LAS 4000 • Simple to adapt to established laboratory protocols due to compatibility with most commercially available Western blotting reagents such as molecular weight markers, membranes, and blocking reagents
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