Amersham™ ECL™ Prime Western Blotting Reagent

Amersham™ ECL™ Prime Western Blotting Reagent
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 4.2/5.0 (4 reviews)

GE Healthcare

Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumpti...read more

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Reviews

Kevin Tong


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Member since 2011

Organization:
Lehigh University
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RATING: 3.6

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 19 Jan 2012

Application Area: Cell Biology
Amersham™ ECL™ Prime Western Blotting Reagent

"Very easy to use and make. Easy directions within the kit. Can be finicky in presence of light."

Read 4 reviews

Narisorn Kitiyanant


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Member since 2011

Organization:
Mahidol University
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 12 Jan 2012

Application Area: Neuroscience
Amersham™ ECL™ Prime Western Blotting Reagent

"This kit is a high sensitivity ECL. Sometimes we dilute it in distilled water before pouring on to the membranes. It needs incubating for 5 minutes and that's it."

MAGALY MARTINEZ-FERRER


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Member since 2011

Organization:
UNIV. OF PUERTO RICO CANCER CTR.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 04 Jul 2011

Amersham™ ECL™ Prime Western Blotting Reagent

"Very easy to use. Incubation of only one minute. Low background."

Weng Yee Leong


Status:


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Member since 2010

Organization:
Erasmus Medical Center
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RATING: 3.6

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 21 Jan 2011

Amersham™ ECL™ Prime Western Blotting Reagent

"Kit is very self explanatory. Very detailed instruction sheet."

Description

 

Chemiluminescent Amersham ECL Prime Western Blotting Reagent is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. Suitable for use with film of CCD camera and a low pg detection level.

The improved performance of Amersham ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Features
• High signal intensity and sensitivity allow the use of highly diluted primary and secondary antibodies with no reduction in sensitivity
• Stable signal allows multiple exposures and large experimental series
• Optimized for use with CCD-based imagers such as ImageQuant™ LAS 4000
• Simple to adapt to established laboratory protocols due to compatibility with most commercially available Western blotting reagents such as molecular weight markers, membranes, and blocking reagents

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