Streptavidin-coated AlphaScreen Donor beads as well as unconjugated AlphaScreen Donor and Acceptor beads are available for any "do-it-yourself" assay development. Conjugation of biomolecules to AlphaScreen beads is a very simple procedure.
Unconjugated beads are readily available to facilitate access to, and allow the creation of, custom assay formats not commercially available or of a proprietary nature. Note that 1 mg translates into 2,000 assay points based on a 25 µL reaction volume and final bead concentration of 20 µg/mL
Kit Contents: Streptavidin Donor beads - 2 tubes each containing 200µL of beads at 5 mg/mL in 25mM HEPES 100mM NaCl pH 7.4.
Unconjugated Acceptor beads - 2 tubes each containing 50µL of beads at 20 mg/mL in 0.1 M MES buffer pH 6.0.