The AequoScreen™ technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. The product portfolio includes: - Parental cell lines...read more
The AequoScreen™ technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. The product portfolio includes:
- Parental cell lines (expressing mitochondrially targeted apoaequorin with / without the promiscuous G-protein Gα16) - Double transfected cell lines (expressing mitochodrially targeted apoaequorin and GPCR with / without the promiscuous G-protein Gα16)
Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
Features and benefits include:
- Fully automatable and miniaturizable to 1536 - Enables reduction in reagents and consumable costs - High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators - Robust assay (Z, CV values, solvent resistance) - Reduces the number of false positives from autofluorescent compounds in screening libraries - Option to work with cells in suspension to increase throughput - Amenable for use with calcium coupled ion channels and tyrosine kinase receptors
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