Polyacrylamide is a commonly used electrophoresis matrix formed by free-radical polymerization of acrylamide and a co-monomer crosslinker. Reliable electrophoresis results depend on the reproducibility of this reaction, making highly-purified starting reagents an important factor in gel quality for uniform polymerization.
Acrylamide is available in three forms to meet the needs if every laboratory
• Acrylamide Powders
Acrylamide and bis-acrylamide powders allow complete adjustment of concentration and acrylamide/bis ratio, to meet all application needs. Powders are available in a variety of package sizes, are economical, and have a shelf life of 1 year or more. They can be stored at room temperature, saving cold-storage space.
• Premixed Acrylamide/Bis Powders
Premixed acrylamide/bis powders contain premeasured amounts of Bio-Rad's 99.9% pure acrylamide and bis-acrylamide. Stock solutions from 30% to 50% can be prepared directly in the bottle by adding the indicated amount of water and stirring. The shelf life of the premixed powders is 1 year at room temperature. After they have been made into solutions, premixed powders should be stored at 4 °C, away from light, for maximum stability.
• Acrylamide Solutions
Bio-Rad's acrylamide solutions are available in two ready-to-use concentrations, 30% and 40%/ Also, in theww acrylamide/bis ratios (19:1, 29:1 and 37.5:1).