ACQUITY UPLC HSS T3 Columns for Polar Compound Retention by Waters

Manufacturer Waters
4.6
/
5.0
  |  13 reviews
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order ... Read more


ACQUITY UPLC HSS T3 Columns for Polar Compound Retention by Waters product image
ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
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Average Rating: 4.6
13 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: Catalent Pharma Solution



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Esay to take care, good and reliable even with harsh phases
Rating: 5.0

  • Application Area: Separation

"Durable, reliable and longlisting. Even when using harsh mobile phases this can last more than 3000 injections"

Review date: 10 Feb 2023 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Universidad Autónoma de Nayarit



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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An excellent tool for analyzing a wide variety of molecules.
Rating: 4.0

  • Application Area:Analysis of secondary metabolites of medicinal plants

"The column is very useful to analyze secondary metabolites of plants, as well as synthesis molecules, mycotoxins, among others. It is easy to create methods and mass chromatograms and spectra come out in good resolution."

Review date: 27 Sep 2022 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Universidad Autónoma de Nayarit



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results and easy to use
Rating: 4.0

  • Application Area:Quantitative and qualitative detection of plant secondary metabolites

"The column is easy and can analyze many products, polar and non polar."

Review date: 16 Dec 2021 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2003

  • Organization: Aristotle University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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A basic column type for polar metabolites
Rating: 5.0

  • Application Area:UPLC column

"One of the most used column types in our lab. We use it for polar metabolites and various specimens. High quality. Very reproducible and efficient."

Review date: 15 Nov 2021 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2003

  • Organization: Aristotle University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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One of our mostly used columns
Rating: 5.0

  • Application Area:UPLC column

"Our gold standard for polar metabolites in metabolomics LC-MS analysis. We base a number of protocols in this column. Might be more expensive than the competition but is high quality."

Review date: 15 Nov 2021 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: CSIR-IHBT



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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A great product worth buying and using. Time and money saver.
Rating: 5.0

  • Application Area:Analysis of metabolites in natural products

"HSS T3 columns are one of the best columns for the separation of mixtures of analytes. Working with natural products and analyzing huge mixtures of compounds is such a tough job but HSS T3 made it easy. Saving time, the separation is now easy as well as cost effective."

Review date: 30 Oct 2020 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: CSIR-IHBT



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Truly, I can say it's great and one of the best.
Rating: 5.0

  • Application Area:Quantification of phenolics/flavonoids & theanine in medicinal plants

"Great resolution and separation achieved by this HSS T3 column. I was struggling in the method development of mixtures of phenolics/flavonoids and Theanine, but after using the HSS T3 column, many of the problems of separating the standard mixture of phenolics and flavonoids were solved, with greater separation from end to end baseline with excellent repeatability. A wide range of compouds are now quantified and separated by this column. Thanks to Waters Corporation. Looking for buy a new one in the next month."

Review date: 24 Jan 2020 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Expert Reviewer

  • Member since: 2013

  • Organization: JRFAmerica



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Very good product
Rating: 4.0

  • Application Area:Analysis of pesticides and biopesticides in all different matrices

"The column is reliable and easy to use. normally there are no clogs or leaking issues and it is very robust"

Review date: 05 Jul 2019 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: The Coca-Cola Company



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Fairly good produt for beverage analysis.
Rating: 3.7

  • Application Area:Food and beverage

"Good product for amino acid sepration and sweetner analysis."

Review date: 26 Mar 2019 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Clinical Phenome Centre



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Brilliant column!
Rating: 5.0

  • Application Area:Metabolic profiling of urine samples

"We love this column. It is robust, reliable and durable. It is one of our favourite standard column to do high-throughput metabolomic profiling analysis. "

Review date: 31 May 2016 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention


  • Status:

    Expert Reviewer

  • Member since: 2012

  • Organization: EUROFINS Biomnis



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Vitamin In Blood Analysis

"This column is efficient and robust. We run on it up to 192 injections (2 plates) a day from whole blood extraction. It is possible to run more than 2000 injections on it. "

Review date: 14 Oct 2015 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: LabAnalysis srl



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.3

  • Application Area:pesticide residues

"Highly versatile, durable, high performance and good durability and reliability. Combined with LC-MS/MS it returns excellent results. "

Review date: 04 Aug 2014 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
  • Status:

    Expert Reviewer

  • Member since: 2013

  • Organization: ONCODESIGN



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Bioanalysis

"The only reverse phase column which can stand 100% water elutes, and if you respective the maximum temperature of 45°C, this column is very robust. It is possible to inject around 5000-6000 samples before any separation capacity is lost. If you just use an in-line frit on the front of the column and this investment will be a good investment. "

Review date: 20 Dec 2013 | ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations.

The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns.

Easier Method Transfer

Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time.

Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.

Product Overview

ACQUITY UPLC HSS T3 Columns for Polar Compound Retention by Waters product image

ACQUITY UPLC HSS T3 Columns for Polar Compound Retention

Manufacturer Waters

4.6 / 5.0 | 13 reviews