ACQUITY UPLC BEH Columns

ACQUITY UPLC BEH Columns
by Waters
 4.2/5.0 (16 reviews)

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid [BEH] particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer ex...read more

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Reviews

AVERAGE RATING: 4.2

16 scientists have reviewed this product

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  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY

Arslan Ali


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Member since 2010

Organization:
Lund University, Sweden / University of Karachi, Pakistan
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 01 May 2014

Application Area: Metabolomics
ACQUITY UPLC BEH Columns

"Waters has introduced Acquity BEH amide (HILIC Lite) columns parallel to other HILIC columns. BEH Amide columns are far superior in stability and robustness then other HILIC columns in waters portfolio. Generally HILIC columns tend to leech polymer into ion source of Mass spectrometers leading to serious contamination. Also HILIC columns are very sensitive to changes in mobile phase buffers. Both of these problems are addressed quiet well by introduction of this new column. Although still lot more to be done to make such column comparable to a good RP-18 column, still they are probably better choice for studying acid and phosphates such as metabolites of Carbon Central Metabolism. "

Read 16 reviews

Boudewijn Hollebrands


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Member since 2013

Organization:
University of Amsterdam
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RATING: 3.3

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 12 Aug 2013

ACQUITY UPLC BEH Columns

"Robust columns, easy to use, easy to install and to implement. Application notes available for a wide range of applications. "

Stijn Hendrickx


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Member since 2013

Organization:
KULeuven
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RATING: 3.7

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 08 Aug 2013

Application Area: 2D-LC-MS
ACQUITY UPLC BEH Columns

"Product is very robust and efficiency is very good. Robustness after more than 1000 injections is still very good. When contacting sales representatives, the follow-up is usually quite good."

Jason Schlaff


Status:


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Member since 2013

Organization:
RTI Laboratories
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 03 Jul 2013

Application Area: Explosives Analysis
ACQUITY UPLC BEH Columns

"This column is both robust and reasonably priced. I have been running EPA 8330B explosive analysis on the same column for months with little sign of degradation. Most importantly, in conjunction with a Waters UPLC, productivity has jumped close to 400% due to runtime reductions from ~45 mins on an HPLC to ~10 mins with this column."

Blake Rushing


Status:


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Member since 2010

Organization:
Catawba College
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 03 Jun 2013

Application Area: Toxicology
ACQUITY UPLC BEH Columns

"Column works well for general use. Gives good separation and has high durability."

Sarah DeCicco


Status:


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Member since 2013

Organization:
Regeneron Pharmaceuticals
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 28 May 2013

Application Area: Protein characterization
ACQUITY UPLC BEH Columns

"This product provides reproducible and reliable chromatograms that are easily used. I would suggest coupling the guard column with the columns at the time of purchase.  Most assays require this and I would find it helpful."

Benjamin Haywood


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Member since 2013

Organization:
St. John Fisher College
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RATING: 3.7

  • 4 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 28 May 2013

ACQUITY UPLC BEH Columns

"The columns and instrument run smoothly and handles very well. User friendly. It is very reliable and I would recommend this instrument to all my friends. The instrument is very user friendly."

Amy Wang


Status:


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Member since 2013

Organization:
Sanofi
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 28 May 2013

Application Area: drug product analysis
ACQUITY UPLC BEH Columns

"It has great separation power for our application. The repeatability is great. We used it for impurity separation and quantification."

Sandy Tang


Status:


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Member since 2013

Organization:
Apotex
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RATING: 3.0

  • 3 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 18 Mar 2013

ACQUITY UPLC BEH Columns

"I am happy with it. The service is fast. We once sent an email with questions and we got a fast reply with a specific answer."

Rupesh Bari


Status:


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Member since 2009

Organization:
Jain Irrigation systems Ltd
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 18 Feb 2012

ACQUITY UPLC BEH Columns

"We are using it on LCMS and find that it gives good separations."

Mohammad Khan


Status:


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Member since 2011

Organization:
King Saud University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 17 Feb 2012

Application Area: Food
ACQUITY UPLC BEH Columns

"I have been using this column since 2010, it has performed excellently."

Raghothama Chaerkady


Status:


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Member since 2007

Organization:
Johns Hopkins University
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 16 Feb 2012

Application Area: Proteomics
ACQUITY UPLC BEH Columns

"BEH columns are useable for all pH range reversed-phase separation and helped us to replace other laborious desalting cleaning steps before LC-MS/MS experiments for peptides. We use it for iTRAQ, phosphopeptide analysis and global proteomic analysis. Peaks are evenly distributed across the gradient unlike strong cation exchange methods. Basic RPLC using BEH column is our favorite offline method. Peptide separation is very different in low and high pH condition offering a scope for better fractionation. The only one pre-step needed is 30 kDa buffer exchange of protein lysate in urea."

Balazs Kormanyos


Status:


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Member since 2011

Organization:
TEVA Pharmaceutical Works Plc
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 18 Aug 2011

ACQUITY UPLC BEH Columns

"I use the BEH C8 50x2.1mm column, I have found them to be really good columns with a long lifetime (20000+ injections)."

Radek Jaźwiec


Status:


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Member since 2006

Organization:
Institute of Sport - Department of Anti-Doping Research
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 20 Oct 2007

ACQUITY UPLC BEH Columns

"It was a revolution in our lab."

Paul Ferguson


Status:


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Member since 2007

Organization:
Pfizer
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RATING: 3.0

  • 3 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 09 Aug 2007

ACQUITY UPLC BEH Columns

Stephen Chesnut


Status:


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Member since 2006

Organization:
Pfizer
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 05 Sep 2006

ACQUITY UPLC BEH Columns

"The columns are changing the way we are doing pharmaceutical development."

Description

 

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid [BEH] particle.

In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms.

BEH Technology particles offer excellent peak shape and efficiency for basic analytes, a rational array of chromatographic selectivity and improvements in chemical stability at mobile phase extremes, particularly at elevated pH.

Application notes and news

Product Overview

ACQUITY UPLC BEH Columns by Waters
ACQUITY UPLC BEH Columns




  • 4out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY





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