ACQUITY UPLC BEH Columns

by Waters

4.3/5.0 (18 reviews)

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid [BEH] particle.

In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms.

BEH Technology particles offer excellent peak shape and efficiency for basic analytes, a rational array of chromatographic selectivity and improvements in chemical stability at mobile phase extremes, particularly at elevated pH.

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Reviews

18 Scientists have reviewed this product


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Average Rating: 4.3

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:18 Feb 2015

  • Application Area:For peptide and small molecule analytical purpose

  • ACQUITY UPLC BEH Columns

"The column very well-kept the reproducibility and the separation robustness even after ten thousand injections. Peak shapes are well-maintained at concentrations very close to lowest limit of quantification, no or very minimum peak smoothing is needed. After-sales cares are very professional and eager to help solving problems. When the column is conjugated with guard column and in-line filter, the life-time and separation reproducibility are ensured. Little expensive in the beginning, but definitely worth it in the long run. "
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:01 May 2014

  • Application Area:Metabolomics

  • ACQUITY UPLC BEH Columns

"Waters has introduced Acquity BEH amide (HILIC Lite) columns parallel to other HILIC columns. BEH Amide columns are far superior in stability and robustness then other HILIC columns in waters portfolio. Generally HILIC columns tend to leech polymer into ion source of Mass spectrometers leading to serious contamination. Also HILIC columns are very sensitive to changes in mobile phase buffers. Both of these problems are addressed quiet well by introduction of this new column. Although still lot more to be done to make such column comparable to a good RP-18 column, still they are probably better choice for studying acid and phosphates such as metabolites of Carbon Central Metabolism. "
Rating: 3.7
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:08 Aug 2013

  • Application Area:2D-LC-MS

  • ACQUITY UPLC BEH Columns

"Product is very robust and efficiency is very good. Robustness after more than 1000 injections is still very good. When contacting sales representatives, the follow-up is usually quite good."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jul 2013

  • Application Area:Explosives Analysis

  • ACQUITY UPLC BEH Columns

"This column is both robust and reasonably priced. I have been running EPA 8330B explosive analysis on the same column for months with little sign of degradation. Most importantly, in conjunction with a Waters UPLC, productivity has jumped close to 400% due to runtime reductions from ~45 mins on an HPLC to ~10 mins with this column."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:28 May 2013

  • Application Area:Protein characterization

  • ACQUITY UPLC BEH Columns

"This product provides reproducible and reliable chromatograms that are easily used. I would suggest coupling the guard column with the columns at the time of purchase.  Most assays require this and I would find it helpful."
Rating: 3.7
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:28 May 2013

  • ACQUITY UPLC BEH Columns

"The columns and instrument run smoothly and handles very well. User friendly. It is very reliable and I would recommend this instrument to all my friends. The instrument is very user friendly."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:28 May 2013

  • Application Area:drug product analysis

  • ACQUITY UPLC BEH Columns

"It has great separation power for our application. The repeatability is great. We used it for impurity separation and quantification."
Rating: 4.6
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:16 Feb 2012

  • Application Area:Proteomics

  • ACQUITY UPLC BEH Columns

"BEH columns are useable for all pH range reversed-phase separation and helped us to replace other laborious desalting cleaning steps before LC-MS/MS experiments for peptides. We use it for iTRAQ, phosphopeptide analysis and global proteomic analysis. Peaks are evenly distributed across the gradient unlike strong cation exchange methods. Basic RPLC using BEH column is our favorite offline method. Peptide separation is very different in low and high pH condition offering a scope for better fractionation. The only one pre-step needed is 30 kDa buffer exchange of protein lysate in urea."

Description

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid [BEH] particle.

In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms.

BEH Technology particles offer excellent peak shape and efficiency for basic analytes, a rational array of chromatographic selectivity and improvements in chemical stability at mobile phase extremes, particularly at elevated pH.

Product Overview

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ACQUITY UPLC BEH Columns

by Waters

4.3/5.0 (18 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from Waters for ACQUITY UPLC BEH Columns