ACQUITY UPLC BEH Columns

Manufacturer Waters
4.3
/
5.0
  |  18 reviews


Product Image
Request Pricing


Turn On Instant Requests

Receive your quote directly from the manufacturer.




Average Rating: 4.3
18 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 4.3

  • Application Area: Metabolomics

"Waters has introduced Acquity BEH amide (HILIC Lite) columns parallel to other HILIC columns. BEH Amide columns are far superior in stability and robustness then other HILIC columns in waters portfolio. Generally HILIC columns tend to leech polymer into ion source of Mass spectrometers leading to serious contamination. Also HILIC columns are very sensitive to changes in mobile phase buffers. Both of these problems are addressed quiet well by introduction of this new column. Although still lot more to be done to make such column comparable to a good RP-18 column, still they are probably better choice for studying acid and phosphates such as metabolites of Carbon Central Metabolism. "

Review date: 22 Apr 2015 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Grupo Bioquimico S.A.



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Rating: 4.3
"We are using Water columns with the aim to develop bioequivalence studies, for different results"

Review date: 18 Feb 2015 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: University of Kansas



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:For peptide and small molecule analytical purpose

"The column very well-kept the reproducibility and the separation robustness even after ten thousand injections. Peak shapes are well-maintained at concentrations very close to lowest limit of quantification, no or very minimum peak smoothing is needed. After-sales cares are very professional and eager to help solving problems. When the column is conjugated with guard column and in-line filter, the life-time and separation reproducibility are ensured. Little expensive in the beginning, but definitely worth it in the long run. "

Review date: 18 Feb 2015 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University of Amsterdam



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.3
"Robust columns, easy to use, easy to install and to implement. Application notes available for a wide range of applications. "

Review date: 12 Aug 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: KULeuven



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7

  • Application Area:2D-LC-MS

"Product is very robust and efficiency is very good. Robustness after more than 1000 injections is still very good. When contacting sales representatives, the follow-up is usually quite good."

Review date: 08 Aug 2013 | ACQUITY UPLC BEH Columns


  • Status:

    Reviewer

  • Member since: 2013

  • Organization: RTI Laboratories



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Explosives Analysis

"This column is both robust and reasonably priced. I have been running EPA 8330B explosive analysis on the same column for months with little sign of degradation. Most importantly, in conjunction with a Waters UPLC, productivity has jumped close to 400% due to runtime reductions from ~45 mins on an HPLC to ~10 mins with this column."

Review date: 03 Jul 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Catawba College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Toxicology

"Column works well for general use. Gives good separation and has high durability."

Review date: 03 Jun 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Regeneron Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Protein characterization

"This product provides reproducible and reliable chromatograms that are easily used. I would suggest coupling the guard column with the columns at the time of purchase.  Most assays require this and I would find it helpful."

Review date: 28 May 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: St. John Fisher College



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.7
"The columns and instrument run smoothly and handles very well. User friendly. It is very reliable and I would recommend this instrument to all my friends. The instrument is very user friendly."

Review date: 28 May 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Sanofi



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:drug product analysis

"It has great separation power for our application. The repeatability is great. We used it for impurity separation and quantification."

Review date: 28 May 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Apotex



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.0
"I am happy with it. The service is fast. We once sent an email with questions and we got a fast reply with a specific answer."

Review date: 18 Mar 2013 | ACQUITY UPLC BEH Columns
  • Status:

    Advanced Reviewer

  • Member since: 2009

  • Organization: Jain Irrigation systems Ltd



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7
"We are using it on LCMS and find that it gives good separations."

Review date: 18 Feb 2012 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: King Saud University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Food

"I have been using this column since 2010, it has performed excellently."

Review date: 17 Feb 2012 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Proteomics

"BEH columns are useable for all pH range reversed-phase separation and helped us to replace other laborious desalting cleaning steps before LC-MS/MS experiments for peptides. We use it for iTRAQ, phosphopeptide analysis and global proteomic analysis. Peaks are evenly distributed across the gradient unlike strong cation exchange methods. Basic RPLC using BEH column is our favorite offline method. Peptide separation is very different in low and high pH condition offering a scope for better fractionation. The only one pre-step needed is 30 kDa buffer exchange of protein lysate in urea."

Review date: 16 Feb 2012 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: TEVA Pharmaceutical Works Plc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"I use the BEH C8 50x2.1mm column, I have found them to be really good columns with a long lifetime (20000+ injections)."

Review date: 18 Aug 2011 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Institute of Sport - Department of Anti-Doping Research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"It was a revolution in our lab."

Review date: 20 Oct 2007 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: Pfizer



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Rating: 3.0

Review date: 09 Aug 2007 | ACQUITY UPLC BEH Columns
  • Status:

    Reviewer

  • Member since: 2006

  • Organization: Pfizer



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"The columns are changing the way we are doing pharmaceutical development."

Review date: 05 Sep 2006 | ACQUITY UPLC BEH Columns
In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid [BEH] particle.

In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms.

BEH Technology particles offer excellent peak shape and efficiency for basic analytes, a rational array of chromatographic selectivity and improvements in chemical stability at mobile phase extremes, particularly at elevated pH.

Product Overview

Product Image

ACQUITY UPLC BEH Columns

Manufacturer Waters

4.3 / 5.0 | 18 reviews