AccuPrimeTM DNA Polymerase System by Thermo Fisher Scientific Invitrogen

AccuPrime™ Taq DNA Polymerase and SuperMixes provide reagents for amplification of nucleic acid templates with antibody-mediated hot-start for improved PCR specificity over other hot-start DNA polymerases (Figure 1). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. AccuPrime™ SuperMix I is optimized for small genomic DNA amplicons (<=200 bp), plasmid DNA, and cDNA templates. AccuPrime™ SuperMix II is optimized for genomic DNA (200 bp-4 kb) templates.

With AccuPrime™ you can:

• Control mispriming at every cycle to get single-band PCR products
• Increase the number of targets in multiplex PCR to save time and reagents
• Eliminate reaction optimization and primer set redesign
• Conveniently assemble reactions at room temperature

Applications:

Multiplex PCR, TOPO TA Cloning® , allele-specific amplifications, and PCR from bacterial colonies in high-throughput applications.