AccuPrime™ Taq DNA Polymerase and SuperMixes provide reagents for amplification of nucleic acid templates with antibody-mediated hot-start for improved PCR specificity over other hot-start DNA polymerases (Figure 1). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. AccuPrime™ SuperMix I is optimized for small genomic DNA amplicons (<=200 bp), plasmid DNA, and cDNA templates. AccuPrime™ SuperMix II is optimized for genomic DNA (200 bp-4 kb) templates.
With AccuPrime™ you can:
Control mispriming at every cycle to get single-band PCR products
Increase the number of targets in multiplex PCR to save time and reagents
Eliminate reaction optimization and primer set redesign
Conveniently assemble reactions at room temperature
Multiplex PCR, TOPO TA Cloning® , allele-specific amplifications, and PCR from bacterial colonies in high-throughput applications.
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