AccuPrime™ Pfx DNA Polymerase by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´5´ exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime™ accessory pro... Read more
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AccuPrime™ Pfx DNA Polymerase
Average Rating: 4.7
2 Scientists have reviewed this product
Status:
Reviewer
Member since: 2014
Organization: Leibniz Institute of Plant Biochemistry- Molecular Signal Processing
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- Ease of use
- After sales service
- Value for money
Rating: 4.3
Application Area: for Site-directed Mutagenesis
"I have been using SDM kits for doing Site-directed Mutagenesis which was really expensive but later started using Pfx enzyme for customized SDM reaction set-up which was comparatively more efficient and reproducible."
Review date: 08 Jan 2015 | AccuPrime™ Pfx DNA Polymerase
Status:
Reviewer Member since: 2009
Organization: LIAI
-
- Ease of use
- After sales service
- Value for money
Rating: 5.0
Review date: 12 Oct 2009 | AccuPrime™ Pfx DNA Polymerase
Review date: 12 Oct 2009 | AccuPrime™ Pfx DNA Polymerase
AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´5´ exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone.
With AccuPrime™ Pfx DNA Polymerase you'll:
With AccuPrime™ Pfx DNA Polymerase you'll:
- Achieve greater than 26 times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 12 kb
- Minimize PCR optimization
Product Overview
