Bioneer’s AccuPrep® Plasmid MiniPrep DNA Extraction Kit allows for the extraction of highly-pure plasmid DNA from as E. coli bacterial cultures. A260/A280 ratio greater than 1.8 and Yields of up to 20 μg of high-copy plasmid DNA can be routinely attained using the AccuPrep Plasmid MiniPrep DNA Extraction Kit. The AccuPrep Plasmid MiniPrep DNA Extraction Kit was developed for plasmid DNA purification from cultured bacterial cells within 20 minutes. The overall principle uses a modified alkaline lysis method. Collected cells are resuspended in Resuspension Buffer. Following the addition of Lysis Buffer and Neutralization Buffer to the lysate, the chromosomal DNA and cell debris will be forms an insoluble aggregate. The insoluble protein aggregate is removed following centrifugation and tran...Read more
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