Bioneer’s AccuPrep® PCR Purification Kit allows rapid purification of DNA fragments (dsDNA and ssDNA) from PCR products and other enzymatic reactions. Purification of up to 10 µg of DNA fragments from PCR and other enzymatic products within 5 minutes with > 90% recovery are routine with the AccuPrep PCR Purification Kit. Size range is 100 bp - 10 kb, so common 20 - 40 mer oligonucleotides are easily removed. Elution volume can be as little as 30 μl when concentrated product is needed. With Bioneer’s PCR cleanup protocol, removal of mineral oil is not necessary. The principle of the AccuPrep PCR Purification Kit is based on the adsorption of DNA in glass filter. Chaotropic salt enhances the binding of DNA onto a glass filter that is fixed in the AccuPrep binding column tube. Adsorption of DNA is very selective so that other proteins and salts are not adsorbed and pass through the glass filter. Washing eliminates salts and other proteins that may bind non-specifically. High-purity DNA fragments are eluted with provided AccuPrep EL Buffer (10 mM Tris-Cl, pH 8.5) or distilled water. Purified DNA fragment can be immediately used for subcloning, sequencing, and other molecular biological applications.
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