Thermo Scientific™ Acclaim™ SEC-300 and SEC-1000 columns are a family of high-performance size-exclusion chromatography columns specifically designed for the separation of water-soluble polymers and oligomers. Acclaim SEC columns are available in two pore sizes, 300 Å and 1000 Å, and are optimized for separating a broad molecular weight range. Water-soluble polymers cover a wide range of highly varied families of products, both of natural or synthetic origin, and have numerous uses in pharmaceuticals, medications, cosmetics, food products, and industrial processes, to name a few.
Column features include:
These columns are packed with spherical, monodispersed, hydrophilic polymethacrylate particles synthesized by multipore particle synthesis technology. Combined with the use of small particle sizes (5 µm for Acclaim SEC-300 and 7 µm for Acclaim SEC-1000), they provide high-resolution and good linearity of the calibration curve for accurate and reliable analysis of a variety of water-soluble polymers.
The Acclaim SEC-1000 uses polymethacrylate particles with a nominal pore size of 1000 Å, resulting in a broad linear calibration range from 1,000 to 1,000,000 Dalton. Therefore, it is suitable for characterizing high molecular weight polymers. The Acclaim SEC-300 uses polymethacrylate particles with a nominal pore size of 300 Å, giving a linear calibration range from 100 to 50,000 Dalton. As a result, this column offers good resolution for lower molecular weight polymers or oligomers.