Thermo Scientific Acclaim® Polar Advantage II (PA2) columns feature a patented bonding chemistry that provides enhanced hydrolytic stability from pH 1.5–10 and allows resolution of a wide variety of polar and nonpolar analytes. The Acclaim PA2 is compatible with 100% aqueous mobile phases and exhibits high reversed-phase capacity, with selectivity complementary to conventional C18 columns such as the Acclaim 120 C18. As with all Acclaim columns, the Acclaim PA2 is LC/MS-compatible.
- Single-run analysis of polar and nonpolar analytes
- Hydrolytically stable (pH 1.5–10)
- Rugged column with high efficiency, symmetrical peak shapes even with basic and acidic compounds
- Compatible with 100% aqueous mobile phases
- High reversed-phase capacity with selectivity complementary to conventional C18 columns
Acclaim PA2 columns are compatible with 100% aqueous mobile phases, overcoming many of the limitations of conventional C8 and C18 reversed-phase columns. Conventional reversed-phase columns are unstable under these conditions and rapidly lose their ability to retain analytes.
Acclaim PA2 columns are available both in the 3- and 5-μm particle sizes and 4.6-, 3.0, and 2.1 mm- diameters, with and average pore diameter of 120Å. Acclaim PA2 is also available in 2.2 µm RSLC format (see Acclaim Rapid Separation LC (RSLC) Column section for more details). Acclaim PA2 may be used for some LC/MS applications.
Highly Stable Under Wide pH Range
The proprietary bonding of the Acclaim PA2 column resists hydrolytic attack by protecting the bonded phase at low pH. This column is highly stable with low pH.
In addition to low pH stability, although it is common to analyze basic compounds under high-pH conditions to reduce peak tailing, most polar-embedded phases are even less hydrolytically stable than conventional C18 columns. The Acclaim PA2 is specifically designed to withstand high pH conditions, making it a good choice for the separation of both basic and acidic analytes.