The Thermo Scientific Acclaim® Mixed-Mode HILIC-1 column utilizes a unique silica-based, mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) properties. The column functional group features a hydrophobic alkyl chain and hydrophilic diol functionality. This combination allows both hydrophobic interaction and hydrophilic interaction to be utilized to optimize separations.

Features:

• Operates in both RP and HILIC modes


• Retains highly polar molecules


• Unique selectivity complementary to RP columns


• Superior performance compared to conventional diol-based columns


• High efficiency column for high resolution separations

The Acclaim Mixed-Mode HILIC-1 columns consist of 5 μm high-purity, porous, spherical silica with 120 Å-diameter pores bonded with proprietry functional groups, which provide unsurpassed resolution and peak symmetry for a variety of polar and nonpolar molecules.

Wide Range of Applications
The Acclaim Mixed-Mode HILIC-1 column separates both polar and nonpolar molecules with selectivity complementary to RP columns and is suitable for a broad range of applications, including drug metabolites, lipids, polyethylene glycols (PEGs), ethoxylated surfactants and more.