Nikon’s A1 MP is a unique multiphoton imaging system featuring a high resolution galvanometer scanner. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.
"Resonance scanning confocal provides very fast 4D imaging. Switch over time between TIRF and confocal is too long to make it useful for live cell dynamic processes. The acquisition software does have some bugs."
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