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Member since: 2014
Organization: Rowan University, School of Osteopathic Medicine
Application Area: cell biology
"Constantly in need of troubleshooting. Would not get another product from this vendor because of the constant need for attention and maintenance, and the trouble with contacting customer care in a timely manner to fix an issue."
Member since: 2015
Organization: University of Michigan
Application Area:Live Cell Imaging
"The Nikon A1S laser scanning confocal microscopy system with accompanying Perfect Focus System enabled us to perform sophisticated live cell and tissue experiments. The Elements software made it easy to perform experiments such as mitochondria trafficking, large format stitching of live cell analysis (Calcein, AM), and Rho FRET biosensors. Nikon provides excellent after-sale care and support and the system is a good value for the money. I would highly recommend this system."
Member since: 2012
Organization: University of Freiburg
"This is a very user-friendly microscope, but the software can sometimes be unreliable and crashes. It has very good optics and the image quality is very good, although scanning is much slower than other machines. Also, it has great spectral detection. Despite the software problems, the Nikon rep is always available and we consistently receive updates to overcome problems."
Nikon confocal microscope system A1+ provides high-resolution imaging of up to 4096 x 4096 pixels with a galvano (non-resonant) scanner. With diverse innovative optical and electronic technologies, such as the VAAS pinhole unit, superior image quality has been achieved.
The A1+'s galvano scanner enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with the newly developed scanner driving and sampling systems, plus image correction technology, high-speed acquisition of 10 fps (512 x 512 pixels) is also possible.
The low-angle incidence method utilized on the dichroic mirrors increases fluorescence efficiency by 30%. By employing the hexagonal pinhole, higher brightness equivalent to that of a circular pinhole is achieved. Nikon's original dual integration signal processing technology (DISP) has been implemented in the image processing circuitry to improve electrical efficiency, resulting in an extremely high S/N ratio.
Nikon has developed an original confocal microscopy VAAS (Virtual Adaptable Aperture System, option) that can eliminate flare while retaining image brightness. Because of the deconvolution of the light that passes through the pinhole and the light that does not pass through the pinhole, acquisition of brighter images with less flare is possible. Different sectionings (slice thicknesses) can also be simulated after image acquisition.
Acquisition of a 32-channel spectral image (512 x 512 pixels) with a single scan in 0.6 second is possible. Moreover, 512 x 32-pixel images can be captured at 24 fps.
Accurate spectral unmixing provides maximum performance in the separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Superior algorithms and high-speed data processing enable real time unmixing during image acquisition.
Three user-defined laser shields allow simultaneous use of four lasers selected from a maximum of nine colors, enabling broader band spectral imaging.
Filter-less intensity adjustment is possible by selecting desired spectral ranges from 32 channels that match the spectrum of the fluorescence probe in use and combining them to perform the filtering function.
NIS—Elements C control software enables integrated control of the confocal imaging system, microscope and peripheral devices with a simple and intuitive interface. Diverse reliable analysis functions are also available.