NEBNext® Small RNA Library Prep Set for SOLiD™ by New England Biolabs Inc.

The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents incl... Read more

NEBNext® Small RNA Library Prep Set for SOLiD™

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NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the SOLiD™ 4.

NEBNext® Small RNA Library Prep Set for SOLiD™ Includes:

NEBNext 3´ Ligation Reaction Buffer (2X)
NEBNext 3´ SR Adaptor 3
NEBNext 5´ SR Adaptor 3
NEBNext 5´ Ligation Reaction Buffer
NEBNext SR RT Primer 3
Murine RNase Inhibitor
NEBNext SR Primer R3
Quick-Load pBR322 MspI-DNA Digest (50 μg/ml)
DNA Gel Elution Buffer (1X)
Linear Acrylamide (10 mg/ml)
TE Buffer
Nuclease-free Water
OneTaq Hot Start 2X Master Mix with Standard Buffer (2X)
NEBNext First Strand Synthesis Reaction Buffer (5X)
NEBNext Index 1 Primer for SOLiD
ProtoScript II Reverse Transcriptase (200,000 units/ml)

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