The Marianas™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for both live and fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering.
Methods: • 7D imaging • FRET • TIRF • FLIM • Ratiometric imaging • Photomanipulation o FRAP o FLIP o Selective illumination o Photoablation o Photoactivation o Photoswitching • High content screening • Rapid 4D (collecting 3D stacks over time by using the streaming capture mode of the camera) • Single molecule studies
Technologies: • SlideBook™ software • Vector™ high speed x,y scanner • Ablate! ™ laser ablation system • Spinning Disk Confocal • LaserStack™ laser launch • mSAC™ spherical aberration correction system • mSwitcher™ galvo-based high speed port switcher • Synchronization - high speed TTL triggering of numerous devices • Fluorescence and transmitted light sources • 3D Deconvolution • Frequency modulated light sources and detectors • Environmental control
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