The Marianas™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for both live and fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering. Methods: • 7D imaging • FRET • TIRF • FLIM • Ratiometric imaging • Photomanipulation o FRAP o FLIP o Selective illumination o Photoablation o Photoactivation o Photoswitching • High content screening • Rapid 4D (collecting 3D stacks over time by using the streaming capture mode of the camera) • Single molecule studies Technologies: • SlideBook™ software • Vector™ high speed x,y scanner • Ablate! ™ laser ablation system • Spinning Disk Confo...Read more
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