The Kit contains the following reagents to make an active stable chromogen solution for use with the enzyme horseradish peroxidase.
12 ml Stable DAB solution, store below 0°C.
6 ml Peroxide solution, store 2 - 8°C
6 ml Buffer Stock solution, store 2 - 8°C.
3,3'-Diaminobenzidine (DAB) is the most commonly used chromogen for demonstrating the presence of horseradish peroxidase in immunohistochemistry or immunoblotting. It produces a stable water/alcohol insoluble reddish brown product. The stable DAB (25X) concentrate supplied is sufficient for making 300 ml working solution.