3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA by Merck
TMB is a substrate for horseradish peroxidase. Develops a soluble blue reaction product that may be read at 370 or 655 nm. For endpoint assays the reaction may be stopped with acid, forming a yellow reaction product that may be read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
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